Interacting Effects of Nitrogen Addition and Mowing on Plant Diversity and Biomass of a Typical Grassland in Inner Mongolia

Abstract

It is unclear how N addition and mowing interactively affect grassland community structure and function. We studied the short-term effects of N addition and mowing on the species diversity and biomass of a typical grassland in Inner Mongolia using a split-plot design, with the whole plot representing the mowing treatment and the subplot representing the N addition rate. Three stubble heights were set at 10 cm (M2), 6 cm (M3) and 3 cm (M4), and a no-mowing treatment (M1) was also included. N addition consisted of five rates, i.e., 0 (N1), 2 (N2), 5 (N3), 10 (N4) and 20 (N5) g N m2.yr−1. Mowing significantly increased species diversity. Mowing with a stubble height of 10 cm (M2) significantly increased both species biomass and community biomass. Heavy mowing (3 cm stubble height, M4) weakened the difference in C. squarrosa biomass caused by N addition. Species richness decreased, while community biomass and Leymus chinensis biomass increased significantly with increasing N addition rate. Mowing alleviated the negative effects of N addition on species richness, and this effect was influenced by stubble height. Community biomass and L. chinensis biomass tended to be stable when N addition was greater than 10 g N m2.yr−1. The N saturation threshold of C. squarrosa biomass was much lower than that of community biomass and L. chinensis biomass. Species richness was negatively correlated with the Gini coefficient and litter production, which indicated that light competition and litter accumulation were important factors affecting the decrease in species richness in our study.

Keywords

• nitrogen addition
• mowing
• species diversity
• biomass
• Inner Mongolia