Assessment of the Viability of Cat Oocytes Subjected to Storage at Different Time Intervals

Abstract

The aim of this work was to investigate prolonged storage at 4˚ C on the survival of cat cumulus oocyte complexes. The ovaries were obtained from 20 domestic cats. The ovaries were evaluated at different time intervals. The viability test for cumulus oocyte complexes was performed at 2 hours, 24 hours and 72 hours after being sampled. Tests for the viability of cumulus oocyte complexes were performed with Neutral red and Trypan blue. Storage conditions for cumulus oocyte complexes are a critical step in establishing fertility conservation protocols in animals, as well as for assisted reproduction.

Keywords

• oocyte viability
• neutral red
• trypan blue