Frontiers in Veterinary Science (Aug 2024)
Protective effects of engineered Lactobacillus johnsonii expressing bovine granulocyte-macrophage colony-stimulating factor on bovine postpartum endometritis
- Jing Guo,
- Jing Guo,
- Jing Guo,
- Xu Cao,
- Xu Cao,
- Xu Cao,
- Zhiqiang Li,
- Zhiqiang Li,
- Zhiqiang Li,
- Caiyu Wang,
- Caiyu Wang,
- Caiyu Wang,
- Chengkun Zhong,
- Chengkun Zhong,
- Chengkun Zhong,
- Simin Wang,
- Simin Wang,
- Simin Wang,
- Zhile Fan,
- Zhile Fan,
- Zhile Fan,
- Jing Zhao,
- Jing Zhao,
- Jing Zhao,
- Jun Wang,
- Jun Wang,
- Jun Wang,
- Yi Fang,
- Yi Fang,
- Yi Fang,
- Hongyu Liu,
- Hongyu Liu,
- Hongyu Liu,
- He Ding,
- He Ding,
- He Ding,
- Xin Ma,
- Xin Ma,
- Xin Ma,
- Wenfa Lu,
- Wenfa Lu,
- Wenfa Lu
Affiliations
- Jing Guo
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Jing Guo
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Jing Guo
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Xu Cao
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Xu Cao
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Xu Cao
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Zhiqiang Li
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Zhiqiang Li
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Zhiqiang Li
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Caiyu Wang
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Caiyu Wang
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Caiyu Wang
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Chengkun Zhong
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Chengkun Zhong
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Chengkun Zhong
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Simin Wang
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Simin Wang
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Simin Wang
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Zhile Fan
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Zhile Fan
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Zhile Fan
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Jing Zhao
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Jing Zhao
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Jing Zhao
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Jun Wang
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Jun Wang
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Jun Wang
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Yi Fang
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Yi Fang
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Yi Fang
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Hongyu Liu
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Hongyu Liu
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Hongyu Liu
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- He Ding
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- He Ding
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- He Ding
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Xin Ma
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Xin Ma
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Xin Ma
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- Wenfa Lu
- Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China
- Wenfa Lu
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, China
- Wenfa Lu
- Jilin Province Key Laboratory of Beef Cattle Germplasm Resources Utilization, Jilin Agricultural University, Changchun, China
- DOI
- https://doi.org/10.3389/fvets.2024.1418091
- Journal volume & issue
-
Vol. 11
Abstract
IntroductionPostpartum endometritis is a prevalent reproductive disorder in bovines, leading to a prolonged open period, infertility, and other complications. While Lactobacillus strains can mitigate these conditions by reducing uterine inflammation, their effectiveness is limited due to a lack of direct anti microbial action and extended treatment duration. This study aimed to construct a recombinant Lactobacillus johnsonii strain expressing bovine Granulocyte-macrophage colony-stimulating factor (GM-CSF) to evaluate its potential in reducing postpartum uterine inflammation.MethodsThe recombinant Lactobacillus johnsonii strain was engineered to express bovine GM-CSF and administered to pregnant mice via vaginal perfusion. Postpartum endometritis was induced using E. coli infection, and the protective effects of the engineered strain were assessed. Inflammatory markers (IL-6, IL-1β, TNF-α), myeloperoxidase (MPO) activity, and nitric oxide (NO) concentration were measured. Histological examination was performed to evaluate uterine morphology and pathological damage.ResultsThe recombinant L. johnsonii strain expressing GM-CSF significantly reduced inflammation levels induced by E. coli infection in the uterus. This reduction was evidenced by decreased expression of IL-6, IL-1β, TNF-α, as well as reduced MPO activity and NO concentration. Histological examination revealed improved uterine morphology and reduced pathological damage in mice treated with the recombinant GM-CSF strain. Crucially, the recombinant strain also exerts beneficial effects on bovine endometritis by reducing levels of inflammatory cytokines, suggesting a beneficial effect on clinical bovine endometritis.ConclusionThe recombinant Lactobacillus johnsonii expressing GM-CSF demonstrated protective effects against postpartum endometritis in bovines by reducing inflammatory cytokines. The findings indicate the potential clinical application of this engineered strain in preventing postpartum uterine inflammation, offering a novel and effective protective option for related disorders and improving bovine reproductive efficiency.
Keywords
