Multifunctional chitosan-based nanocomposite microgels for reconstructing osteochondral defects

Yifan Lu; Ze-Yu Wang; Jianhong Xiao; Zhengyi Zhao; Xiaoyu Liao; Huai-Ling Gao; Duohong Zou

Materials & Design (Mar 2024)

Multifunctional chitosan-based nanocomposite microgels for reconstructing osteochondral defects

Yifan Lu,
Ze-Yu Wang,
Jianhong Xiao,
Zhengyi Zhao,
Xiaoyu Liao,
Huai-Ling Gao,
Duohong Zou

Affiliations

Yifan Lu: National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai, Research Institute of Stomatology, Department of Oral Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; Department of Dental Implant Center, Stomatology Hospital & College, Key Laboratory of Oral Diseases Research of Anhui Province, Anhui Medical University, Hefei 230032, China
Ze-Yu Wang: Department of Chemistry, Institute of Biomimetic Materials & Chemistry, Anhui Engineering Laboratory of Biomimetic Materials, Division of Nanomaterials & Chemistry, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China
Jianhong Xiao: National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai, Research Institute of Stomatology, Department of Oral Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; Department of Dental Implant Center, Stomatology Hospital & College, Key Laboratory of Oral Diseases Research of Anhui Province, Anhui Medical University, Hefei 230032, China
Zhengyi Zhao: National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai, Research Institute of Stomatology, Department of Oral Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; Department of Dental Implant Center, Stomatology Hospital & College, Key Laboratory of Oral Diseases Research of Anhui Province, Anhui Medical University, Hefei 230032, China
Xiaoyu Liao: National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai, Research Institute of Stomatology, Department of Oral Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; Department of Dental Implant Center, Stomatology Hospital & College, Key Laboratory of Oral Diseases Research of Anhui Province, Anhui Medical University, Hefei 230032, China
Huai-Ling Gao: Department of Chemistry, Institute of Biomimetic Materials & Chemistry, Anhui Engineering Laboratory of Biomimetic Materials, Division of Nanomaterials & Chemistry, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China; CAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, University of Science and Technology of China, Hefei, 230027 China; Corresponding authors at: CAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, University of Science and Technology of China, Hefei, 230027 China (H.-L. Gao), National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai, Research Institute of Stomatology, Department of Oral Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China (D. Zou).
Duohong Zou: National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai, Research Institute of Stomatology, Department of Oral Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; Department of Dental Implant Center, Stomatology Hospital & College, Key Laboratory of Oral Diseases Research of Anhui Province, Anhui Medical University, Hefei 230032, China; Corresponding authors at: CAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, University of Science and Technology of China, Hefei, 230027 China (H.-L. Gao), National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai, Research Institute of Stomatology, Department of Oral Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China (D. Zou).

Journal volume & issue: Vol. 239
p. 112821

Abstract

Read online

Treatment of osteochondral (OC) defects is still an immense challenge in clinic due to lack of satisfactory strategy to simultaneously regenerate both cartilage and subchondral bone. Herein, we propose an effective bottom-up strategy by developing a type of multifunctional chitosan-based nanocomposite microgels as the building blocks to repair OC defects. The microgels are biocompatible, and biodegradable, and have a hierarchical nanofibrous structure, mimicking the structural feature of extracellular matrix (ECM) to facilitate cell attachment and proliferation. Additionally, β-tricalcium phosphate (β-TCP) nanoparticles are incorporated in the microgels not only to promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), but also to facilitate the loading of transforming growth factor beta 1 (TGF-β1). For reconstructing OC defects, pure microgels are implanted at the bottom layer of the OC defects in rat knee joints to promote osteogenesis, while the microgels loaded with TGF-β1 are implanted at the top layer of the OC defects to promote cartilage regeneration. This modular microgel system results in simultaneous regeneration of cartilage and subchondral bone in OC defects, which is expected as a promising way for repairing other complex tissue defects in the field of tissue engineering.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

About the journal

Abstract

Keywords