Blood-brain-barrier spheroids as an in vitro screening platform for brain-penetrating agents

Choi-Fong Cho; Justin M. Wolfe; Colin M. Fadzen; David Calligaris; Kalvis Hornburg; E. Antonio Chiocca; Nathalie Y. R. Agar; Bradley L. Pentelute; Sean E. Lawler

doi:10.1038/ncomms15623

Nature Communications (Jun 2017)

Blood-brain-barrier spheroids as an in vitro screening platform for brain-penetrating agents

Choi-Fong Cho,
Justin M. Wolfe,
Colin M. Fadzen,
David Calligaris,
Kalvis Hornburg,
E. Antonio Chiocca,
Nathalie Y. R. Agar,
Bradley L. Pentelute,
Sean E. Lawler

Affiliations

Choi-Fong Cho: Department of Neurosurgery, Harvey Cushing Neuro-Oncology Laboratories, Brigham and Women’s Hospital, Harvard Medical School
Justin M. Wolfe: Department of Chemistry, Massachusetts Institute of Technology
Colin M. Fadzen: Department of Chemistry, Massachusetts Institute of Technology
David Calligaris: Department of Neurosurgery and Radiology, Brigham and Women’s Hospital, Harvard Medical School
Kalvis Hornburg: Department of Neurosurgery, Harvey Cushing Neuro-Oncology Laboratories, Brigham and Women’s Hospital, Harvard Medical School
E. Antonio Chiocca: Department of Neurosurgery, Harvey Cushing Neuro-Oncology Laboratories, Brigham and Women’s Hospital, Harvard Medical School
Nathalie Y. R. Agar: Department of Neurosurgery and Radiology, Brigham and Women’s Hospital, Harvard Medical School
Bradley L. Pentelute: Department of Chemistry, Massachusetts Institute of Technology
Sean E. Lawler: Department of Neurosurgery, Harvey Cushing Neuro-Oncology Laboratories, Brigham and Women’s Hospital, Harvard Medical School

DOI: https://doi.org/10.1038/ncomms15623
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 14

Abstract

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In vitroblood-brain barrier (BBB) models are crucial tools for screening brain-penetrating compounds. Here the authors develop a self-assembling BBB spheroid model with superior performance to the standard transwell BBB model, and use their platform to identify cell-penetrating peptides that can cross the BBB.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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