Frontiers in Cellular Neuroscience (Oct 2023)

Targeted approaches to delineate neuronal morphology during early development

  • Bimin Liu,
  • Bimin Liu,
  • Yuxiao Li,
  • Yuxiao Li,
  • Miao Ren,
  • Miao Ren,
  • Xiangning Li,
  • Xiangning Li,
  • Xiangning Li

DOI
https://doi.org/10.3389/fncel.2023.1259360
Journal volume & issue
Vol. 17

Abstract

Read online

Understanding the developmental changes that affect neurons is a key step in exploring the assembly and maturation of neural circuits in the brain. For decades, researchers have used a number of labeling techniques to visualize neuronal morphology at different stages of development. However, the efficiency and accuracy of neuronal labeling technologies are limited by the complexity and fragility of neonatal brains. In this review, we illustrate the various labeling techniques utilized for examining the neurogenesis and morphological changes occurring during the early stages of development. We compare the advantages and limitations of each technique from different aspects. Then, we highlight the gaps remaining in our understanding of the structure of neurons in the neonatal mouse brain.

Keywords