Pre-Treatment with Grape Seed Extract Reduces Inflammatory Response and Oxidative Stress Induced by <i>Helicobacter pylori</i> Infection in Human Gastric Epithelial Cells
Jose Manuel Silvan,
Alba Gutierrez-Docio,
Esperanza Guerrero-Hurtado,
Lucia Domingo-Serrano,
Ana Blanco-Suarez,
Marin Prodanov,
Teresa Alarcon-Cavero,
Adolfo J. Martinez-Rodriguez
Affiliations
Jose Manuel Silvan
Microbiology and Food Biocatalysis Group (MICROBIO), Department of Biotechnology and Food Microbiology, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolás Cabrera, 9. Campus de Cantoblanco, Universidad Autonoma de Madrid, 28049 Madrid, Spain
Alba Gutierrez-Docio
Department of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autonoma de Madrid, 28049 Madrid, Spain
Esperanza Guerrero-Hurtado
Department of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autonoma de Madrid, 28049 Madrid, Spain
Lucia Domingo-Serrano
Microbiology and Food Biocatalysis Group (MICROBIO), Department of Biotechnology and Food Microbiology, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolás Cabrera, 9. Campus de Cantoblanco, Universidad Autonoma de Madrid, 28049 Madrid, Spain
Ana Blanco-Suarez
Microbiology Department, Sanitaria Princesa Research Institute, Hospital Universitario de La Princesa, 28006 Madrid, Spain
Marin Prodanov
Department of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autonoma de Madrid, 28049 Madrid, Spain
Teresa Alarcon-Cavero
Microbiology Department, Sanitaria Princesa Research Institute, Hospital Universitario de La Princesa, 28006 Madrid, Spain
Adolfo J. Martinez-Rodriguez
Microbiology and Food Biocatalysis Group (MICROBIO), Department of Biotechnology and Food Microbiology, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolás Cabrera, 9. Campus de Cantoblanco, Universidad Autonoma de Madrid, 28049 Madrid, Spain
Helicobacter pylori (H. pylori) is a pathogenic bacteria identified as a potential risk factor for gastritis, gastric ulcers and gastric cancer. During the stomach colonization, H. pylori triggers a strong inflammatory response and subsequent oxidative stress, which are associated with tissue damage. For this reason, it is of particular interest to develop alternative natural tools that enable modulation of the associated damaging immune response. With this purpose, we obtained grape seed extract (GSE) from sweet (not fermented) food grade seeds. The aim of our study was to investigate the effect of GSE and its two enriched procyanidins fractions (OPC and PPC) on the inflammatory process and oxidative stress produced by different H. pylori strains in human gastric epithelial cells (AGS). Anti-inflammatory activity was evaluated by measuring the level of interleukin-8 (IL-8) secretion. IL-8 production was significantly reduced in H. pylori-infected human gastric epithelial cells pre-treated with GSE or its enriched fractions when compared with non-pre-treated infected cells (from 21.6% to 87.8%). Pre-treatment with GSE or its fractions significantly decreased intracellular reactive oxygen species (ROS) production in AGS cells after infection, depending on the H. pylori strain. Our results also showed that GSE and its fractions demonstrate antibacterial activity against all strains of H. pylori used in the study. This work demonstrates the effectiveness of GSE enriched in procyanidins against the main events associated with H. pylori infection.
