Comparative performance of liquid chromatography and spectrophotometry in determining metformin hydrochloride within pharmaceutical formulations
Amani Kurdi,
Waleed Alhussaini,
Abdulrahman Alawaji,
Abdullah Alhudathi,
Rakan Alharbi,
Faisal Binsaleh,
Yazeed Alghamidi,
Abdulkareem Al Bekairy,
Abdulmalik Alkatheri,
Imadul Islam,
Ibrahim Farh,
Ezzeldeen Ghanem,
Mahmoud Mansour
Affiliations
Amani Kurdi
Department of Pharmaceutical Analysis, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Waleed Alhussaini
Department of Pharmaceutical Analysis, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Abdulrahman Alawaji
College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Abdullah Alhudathi
College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Rakan Alharbi
College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Faisal Binsaleh
College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Yazeed Alghamidi
College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Abdulkareem Al Bekairy
College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Abdulmalik Alkatheri
College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Imadul Islam
Department of Pharmaceutical Analysis, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Ibrahim Farh
College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Ezzeldeen Ghanem
Department of Pharmaceutical Analysis, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia
Mahmoud Mansour
College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of the National Guard - Health Affairs, Riyadh, Saudi Arabia; Corresponding author. College of Pharmacy, King Saud Bin Abdul-Aziz University for Health Sciences (KSAU-HS), PO BOX 3660, Riyadh, 11481, Kingdom of Saudi Arabia.
The present study compared the performance of Ultra-high performance liquid chromatography (UHPLC) and UV–Vis spectrophotometry for the quantification of metformin hydrochloride in five commercially available metformin hydrochloride products with different strengths. The metformin hydrochloride was measured in the UHPLC with a mobile phase consisting of a mixture of 0.05 M phosphate buffer solution and methanol (35:65, v/v) with a pH of 3.6. Metformin hydrochloride was determined spectrophotometrically at 234 nm using a mixture of methanol and water as a blank. The methods’ linearity for metformin hydrochloride was within the concentration range of (2.5–40 μg/ml) in both techniques. The validation process encompassed assessments of specificity, selectivity, linearity, accuracy, precision, the lower limit of quantification (LLOQ), the lower limit of detection (LLOD), robustness, and system suitability. For the UHPLC validation method, the repeatability and reproducibility (expressed as relative standard deviation) were less than 1.578 and 2.718 %, respectively. The LLOQ for metformin hydrochloride was 0.625 μg/ml, and the LLOD was 0.156 μg/ml. For the UV–Vis spectrophotometric validation method, the repeatability and reproducibility (stated as relative standard deviation) were less than 3.773 and 1.988 %, respectively. The percentage recovery results for the five brands of metformin hydrochloride tablets were (98–101 %) and (92–104 %) for the UHPLC and UV–Vis spectrophotometric methods, respectively. In conclusion, the described methodologies were successfully employed for the quantitative analysis of metformin hydrochloride in different pharmaceutical tablet products.