Assessment of the Prevalence and Drug Susceptibility of <i>Listeria monocytogenes</i> Strains Isolated from Various Types of Meat
Krzysztof Skowron,
Ewa Wałecka-Zacharska,
Natalia Wiktorczyk-Kapischke,
Karolina Jadwiga Skowron,
Katarzyna Grudlewska-Buda,
Justyna Bauza-Kaszewska,
Zuzanna Bernaciak,
Miłosz Borkowski,
Eugenia Gospodarek-Komkowska
Affiliations
Krzysztof Skowron
Department of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, Poland
Ewa Wałecka-Zacharska
Department of Food Hygiene and Consumer Health, Wrocław University of Environmental and Life Sciences, 31 C.K. Norwida St., 50-375 Wrocław, Poland
Natalia Wiktorczyk-Kapischke
Department of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, Poland
Karolina Jadwiga Skowron
Institute of Telecommunications and Computer Science, UTP University of Science and Technology, Al. Prof. S. Kaliskiego 7, 85-796 Bydgoszcz, Poland
Katarzyna Grudlewska-Buda
Department of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, Poland
Justyna Bauza-Kaszewska
Department of Microbiology and Food Technology, UTP University of Science and Technology, 6 Bernardyńska St., 85-029 Bydgoszcz, Poland
Zuzanna Bernaciak
Department of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, Poland
Miłosz Borkowski
Department of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, Poland
Eugenia Gospodarek-Komkowska
Department of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, Poland
Listeria monocytogenes are the etiological factor of listeriosis, and their main source for humans is food. The aim of the current study was to assess the contamination of various types of meat and the drug susceptibility of isolated L. monocytogenes. Between 2016–2018, 6000 swabs were taken (2000 annually) from the surface of pork, beef, and poultry. The analysis of intermediate and finished product samples was carried out in accordance with ISO 11290-1 (International Organization for Standardization). The genetic similarity assessment of the isolates obtained was based on the Pulsed Field Gel Electrophoresis (PFGE) method, and drug-sensitivity assessment using the disc-diffusion method. We found 2.1% of collected samples were L. monocytogenes positive. The level of meat contamination varied depending on its matrix. Most L. monocytogenes were isolated from poultry. It was shown that 39 (32.5%) strains were sensitive to all tested antibiotics and eight (6.7%) were resistant to all five tested antimicrobials. Most strains tested were resistant to cotrimoxazole (55; 45.8%) and meropenem (52; 43.3%), followed by erythromycin (48; 40.0%), penicillin (31; 25.8%), and ampicillin (21; 17.5%). High prevalence of this pathogen may be a serious problem, especially when linked with antibiotic resistance and high percentage of serotypes responsible for listeriosis outbreaks.
