Isolation of Microsatellite Markers from De Novo Whole Genome Sequences of <i>Coptotermes gestroi</i> (Wasmann) (Blattodea: Rhinotermitidae)

Abstract

Coptotermes gestroi (Wasmann) (Blattodea: Rhinotermitidae) is a subterranean termite species from Southeast Asia which has been unintentionally introduced to many parts of the world through commerce and modern transportation. Known for causing extensive damage to timber used in the built environment, the termite also has a habit of nesting in carton nests in wood and wooden structures in buildings. As so little is known of its breeding system, colony, and genetic structure, we initiated work to sequence its genome with an Illumina HiSeq™ 2000 sequencer. In this publication, we announce our paired-end sequencing data and report the isolation of 119,190 microsatellite markers from our DNA assembly. The microsatellite marker reported in this publication can be used to elucidate the mating system and genetic structure of this highly invasive termite species. Additionally, in this announcement the study authors make the Bio Project sequence accession number SRR13105492 accessible from the Sequence Read Archive database.

Keywords

• <i>Coptotermes gestroi</i>
• microsatellites
• termites
• genomic DNA
• whole-genome sequencing
• Illumina HiSeq^™ 2000