Protocol for T-cell Adhesion Strength on Tumor Cells under Flow Conditions
Marie Boutet,
Katarzyna Franciszkiewicz,
Audrey Floc’h,
Fathia Mami-Chouaib
Affiliations
Marie Boutet
INSERM (Institut National de la Santé et de la Recherche Médicale) U753, Team 1: Tumor Antigens and T-cell Reactivity, Gustave Roussy (Institut de Cancérologie Gustave Roussy), Villejuif, France
Katarzyna Franciszkiewicz
INSERM (Institut National de la Santé et de la Recherche Médicale) U753, Team 1: Tumor Antigens and T-cell Reactivity, Gustave Roussy (Institut de Cancérologie Gustave Roussy), Villejuif, France
Audrey Floc’h
INSERM (Institut National de la Santé et de la Recherche Médicale) U753, Team 1: Tumor Antigens and T-cell Reactivity, Gustave Roussy (Institut de Cancérologie Gustave Roussy), Villejuif, France
Fathia Mami-Chouaib
INSERM (Institut National de la Santé et de la Recherche Médicale) U753, Team 1: Tumor Antigens and T-cell Reactivity, Gustave Roussy (Institut de Cancérologie Gustave Roussy), Villejuif, France
This method allows evaluating the relative adhesion strength between T lymphocytes and specific adherent target cells using a shear force in flow chambers. It is based on the measure of the resistance of conjugates formed between T cells and adherent tumor cells to shear stress in a microfluidic system. For this purpose, T cells, stained with a CellTracker probe, are added into flow channels containing a monolayer of adherent target cells and their progressive detachment under a constant shear stress is then recorded using a fluorescent microscope.
