Syndecan-1 shedding destroys epithelial adherens junctions through STAT3 after renal ischemia/reperfusion injury

Man Guo; Daoqi Shen; Yiqi Su; Jiarui Xu; Shuan Zhao; Weidong Zhang; Yaqiong Wang; Wuhua Jiang; Jialin Wang; Xuemei Geng; Xiaoqiang Ding; Xialian Xu

iScience (Nov 2023)

Syndecan-1 shedding destroys epithelial adherens junctions through STAT3 after renal ischemia/reperfusion injury

Man Guo,
Daoqi Shen,
Yiqi Su,
Jiarui Xu,
Shuan Zhao,
Weidong Zhang,
Yaqiong Wang,
Wuhua Jiang,
Jialin Wang,
Xuemei Geng,
Xiaoqiang Ding,
Xialian Xu

Affiliations

Man Guo: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China
Daoqi Shen: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China
Yiqi Su: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China
Jiarui Xu: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China
Shuan Zhao: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China
Weidong Zhang: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China
Yaqiong Wang: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China
Wuhua Jiang: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China
Jialin Wang: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China
Xuemei Geng: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China
Xiaoqiang Ding: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China; Corresponding author
Xialian Xu: Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Medical Center of Kidney Disease, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China; Key Laboratory of Kidney and Blood Purification, Shanghai, China; Hemodialysis quality control center of Shanghai, Shanghai, China; Corresponding author

Journal volume & issue: Vol. 26, no. 11
p. 108211

Abstract

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Summary: Adherens junctions between tubular epithelial cells are disrupted in renal ischemia/reperfusion (I/R) injury. Syndecan-1 (SDC-1) is involved in maintaining cell morphology. We aimed to study the role of SDC-1 shedding induced by renal I/R in the destruction of intracellular adherens junctions. We found that SDC-1 shedding was increased while the expression of E-cadherin was decreased. This observation was accompanied by the activation of STAT3 in the kidneys. Inhibiting the shedding of SDC-1 induced by I/R could alleviate this effect. Mild renal I/R could induce more severe renal injury, lower E-cadherin expression, damaged cell junctions, and activated STAT3 in knockout mice with the tubule-specific deletion of SDC-1 mice. The results in vitro were consistent with those in vivo. Inhibiting the shedding of SDC-1 could alleviate the decreased expression of E-cadherin and damage of cell adherens junctions through inhibiting the activation of STAT3 during ischemic acute kidney injury.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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