Nature Communications (Sep 2020)

A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells

  • Laura I. FitzGerald,
  • Luigi Aurelio,
  • Moore Chen,
  • Daniel Yuen,
  • Joshua J. Rennick,
  • Bim Graham,
  • Angus P. R. Johnston

DOI
https://doi.org/10.1038/s41467-020-18082-8
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 13

Abstract

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Determining the trafficking of intracellular material is commonly done by colocalisation analysis using microscopy. Here the authors monitor trafficking of select cargo by measuring the conversion of quenched SNAP-tag substrates by subcellularly-localised SNAP-tag and detection by flow cytometry.