Cell-type and subcellular compartment-specific APEX2 proximity labeling reveals activity-dependent nuclear proteome dynamics in the striatum

V. Dumrongprechachan; R. B. Salisbury; G. Soto; M. Kumar; M. L. MacDonald; Y. Kozorovitskiy

doi:10.1038/s41467-021-25144-y

Nature Communications (Aug 2021)

Cell-type and subcellular compartment-specific APEX2 proximity labeling reveals activity-dependent nuclear proteome dynamics in the striatum

V. Dumrongprechachan,
R. B. Salisbury,
G. Soto,
M. Kumar,
M. L. MacDonald,
Y. Kozorovitskiy

Affiliations

V. Dumrongprechachan: Department of Neurobiology, Northwestern University
R. B. Salisbury: Department of Psychiatry, University of Pittsburgh
G. Soto: Department of Neurobiology, Northwestern University
M. Kumar: Department of Neurobiology, Northwestern University
M. L. MacDonald: Department of Psychiatry, University of Pittsburgh
Y. Kozorovitskiy: Department of Neurobiology, Northwestern University

DOI: https://doi.org/10.1038/s41467-021-25144-y
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 16

Abstract

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Mapping neuronal proteomes with genetic, subcellular, and temporal specificity is a challenging task. This study uncovers proteome dynamics in two classes of striatal spiny projection neurons in the mouse brain using a genetically targeted APEX2-based proximity labeling approach.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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