Recombinant Strains Application for Simultaneous Preparation of Several Purified Cholera Vibrio Antigens

Abstract

Proposed is the method for simultaneous isolation and purification of three cholera vibrio key pathogenicity factors (cholera toxin, toxin-co-regulated adhesion pili, O1 antigen Ogawa or Inaba), which are main protective antigens, from previously constructed recombinant Vibrio cholerae strains 2415 Inaba and KM 206 Ogawa. Immunologic activity of the preparations was confirmed using polyclonal high-active specific antisera. Purified antigens can be used for creation and improvement of prophylactic and diagnostic preparations.

Keywords

• recombinant <i>vibrio cholerae</i> strains
• protective antigens
• purification
• antisera
• specificity