Similarities and Differences in Extracellular Vesicle Profiles between Ischaemic Stroke and Myocardial Infarction
Laura Otero-Ortega,
Elisa Alonso-López,
María Pérez-Mato,
Fernando Laso-García,
Mari Carmen Gómez-de Frutos,
Luke Diekhorst,
María Laura García-Bermejo,
Elisa Conde-Moreno,
Blanca Fuentes,
María Alonso de Leciñana,
Eduardo Armada,
Lorena Buiza-Palomino,
Exuperio Díez-Tejedor,
María Gutiérrez-Fernández
Affiliations
Laura Otero-Ortega
Neurological Sciences and Cerebrovascular Research Laboratory, Department of Neurology and Stroke Centre, Neuroscience Area of IdiPAZ, Hospital La Paz Institute for Health Research–IdiPAZ, La Paz University Hospital, Universidad Autónoma de Madrid, 28046 Madrid, Spain
Elisa Alonso-López
Neurological Sciences and Cerebrovascular Research Laboratory, Department of Neurology and Stroke Centre, Neuroscience Area of IdiPAZ, Hospital La Paz Institute for Health Research–IdiPAZ, La Paz University Hospital, Universidad Autónoma de Madrid, 28046 Madrid, Spain
María Pérez-Mato
Neurological Sciences and Cerebrovascular Research Laboratory, Department of Neurology and Stroke Centre, Neuroscience Area of IdiPAZ, Hospital La Paz Institute for Health Research–IdiPAZ, La Paz University Hospital, Universidad Autónoma de Madrid, 28046 Madrid, Spain
Fernando Laso-García
Neurological Sciences and Cerebrovascular Research Laboratory, Department of Neurology and Stroke Centre, Neuroscience Area of IdiPAZ, Hospital La Paz Institute for Health Research–IdiPAZ, La Paz University Hospital, Universidad Autónoma de Madrid, 28046 Madrid, Spain
Mari Carmen Gómez-de Frutos
Neurological Sciences and Cerebrovascular Research Laboratory, Department of Neurology and Stroke Centre, Neuroscience Area of IdiPAZ, Hospital La Paz Institute for Health Research–IdiPAZ, La Paz University Hospital, Universidad Autónoma de Madrid, 28046 Madrid, Spain
Luke Diekhorst
Neurological Sciences and Cerebrovascular Research Laboratory, Department of Neurology and Stroke Centre, Neuroscience Area of IdiPAZ, Hospital La Paz Institute for Health Research–IdiPAZ, La Paz University Hospital, Universidad Autónoma de Madrid, 28046 Madrid, Spain
María Laura García-Bermejo
Biomarkers and Therapeutic Targets Unit, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034 Madrid, Spain
Elisa Conde-Moreno
Biomarkers and Therapeutic Targets Unit, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034 Madrid, Spain
Blanca Fuentes
Neurological Sciences and Cerebrovascular Research Laboratory, Department of Neurology and Stroke Centre, Neuroscience Area of IdiPAZ, Hospital La Paz Institute for Health Research–IdiPAZ, La Paz University Hospital, Universidad Autónoma de Madrid, 28046 Madrid, Spain
María Alonso de Leciñana
Neurological Sciences and Cerebrovascular Research Laboratory, Department of Neurology and Stroke Centre, Neuroscience Area of IdiPAZ, Hospital La Paz Institute for Health Research–IdiPAZ, La Paz University Hospital, Universidad Autónoma de Madrid, 28046 Madrid, Spain
Eduardo Armada
Acute Cardiac Care Unit, Cardiology Department, IdiPAZ Health Research Institute, 28046 Madrid, Spain
Lorena Buiza-Palomino
Clinical Analysis Department, La Paz University Hospital, 28046 Madrid, Spain
Exuperio Díez-Tejedor
Neurological Sciences and Cerebrovascular Research Laboratory, Department of Neurology and Stroke Centre, Neuroscience Area of IdiPAZ, Hospital La Paz Institute for Health Research–IdiPAZ, La Paz University Hospital, Universidad Autónoma de Madrid, 28046 Madrid, Spain
María Gutiérrez-Fernández
Neurological Sciences and Cerebrovascular Research Laboratory, Department of Neurology and Stroke Centre, Neuroscience Area of IdiPAZ, Hospital La Paz Institute for Health Research–IdiPAZ, La Paz University Hospital, Universidad Autónoma de Madrid, 28046 Madrid, Spain
Extracellular vesicles (EVs) are involved in intercellular signalling through the transfer of molecules during physiological and pathological conditions, such as ischaemic disease. EVs might therefore play a role in ischaemic stroke (IS) and myocardial infarction (MI). In the present study, we analysed the similarities and differences in the content of circulating EVs in patients with IS and MI. This prospective observational study enrolled 140 participants (81 patients with IS, 37 with MI and 22 healthy controls [HCs]). We analysed the protein and microRNA content from EVs using proteomics and reverse transcription quantitative real-time polymerase chain reaction and compared it between the groups. In the patients with IS and MI, we identified 14 common proteins. When comparing IS and MI, we found differences in the protein profiles (apolipoprotein B, alpha-2-macroglobulin, fibronectin). We also found lower levels of miR-340 and miR-424 and higher levels of miR-29b in the patients with IS and MI compared with the HCs. Lastly, we found higher miR-340 levels in IS than in MI. In conclusion, proteomic and miRNA analyses suggest a relationship between circulating EV content and the patient’s disease state. Although IS and MI affect different organs (brain and heart) with distinct histological characteristics, certain EV proteins and miRNAs appear to participate in both diseases, while others are present only in patients with IS.