Antioxidant Activity of New Aramide Nanoparticles Containing Redox-Active <em>N</em>-phthaloyl Valine Moieties in the Hepatic Cytochrome P<sub>450</sub> System in Male Rats

Abstract

We report the synthesis of aramide nanoparticles containing a chiral <em>N</em>-phthaloyl valine moiety and their antioxidant activities on hepatic contents of cytochrome P₄₅₀, amidopyrene <em>N</em>-demethylase, aniline-4-hyroxylase and induced the hepatic content of cytochrome b5 and nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome C-reductase. Polymers were obtained as well-separated spherical nanoparticles while highly aggregated particles via H-bonding organization of the aramide-containing pyridine led to a thin layer formation. The effects of the nanoparticles and CCl₄ on enzyme activities and thiobarbituric acid reactive substances (TBARS) levels of male rat liver were studied. Pretreatments of rats with the polyamides prior to the administration of CCl₄ decreased the hepatic content of the tested enzymes. Doses reduced the toxic effects exerted by (•CCl₃) upon the liver through inhibition of the cytochrome P₄₅₀ system. Inhibition of such metabolizing enzymes could reduce the carcinogenic effects of chemical carcinogens.

Keywords

• aramide nanoparticles
• thermal analysis
• valine
• cytochrome P&lt;sub&gt;450&lt;/sub&gt; enzymes
• TBARS