A flow platform for degradation-free CuAAC bioconjugation

Abstract

Cu-catalyzed azide-alkyne cycloaddition (CuAAC) reaction is a common bioconjugation technique; however oxidative degradation and residual copper may limit its use. Here, the authors show that ppm levels of Cu, generated in situ by solvent-mediated pipe erosion in flow, catalyze the CuAAC reaction with no product degradation.