Discrepancy between Mtb-specific IFN-γ and IgG responses in HIV-positive people with low CD4 countsResearch in context
Maphe Mthembu,
Kathryn A. Bowman,
Leela R.L. Davies,
Sharon Khuzwayo,
Lusanda Mazibuko,
Thierry Bassett,
Dirhona Ramjit,
Zoey Mhlane,
Farina Karim,
Galit Alter,
Thumbi Ndung'u,
Emily B. Wong
Affiliations
Maphe Mthembu
Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Kathryn A. Bowman
Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Leela R.L. Davies
Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Sharon Khuzwayo
Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Lusanda Mazibuko
Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Thierry Bassett
Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Dirhona Ramjit
Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Zoey Mhlane
Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Farina Karim
Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Galit Alter
Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Thumbi Ndung'u
Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Emily B. Wong
Corresponding author.; Africa Health Research Institute, Durban, South Africa; University of KwaZulu-Natal, Durban, South Africa; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; Division of Infection and Immunity, University College London, London, United Kingdom; Division of Infectious Diseases, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA
Summary: Background: Tuberculosis (TB) is a leading infectious cause of death worldwide and treating latent TB infection (LTBI) with TB preventative therapy is a global priority. This study aimed to measure interferon gamma (IFN-γ) release assay (IGRA) positivity (the current reference standard for LTBI diagnosis) and Mtb-specific IgG antibodies in otherwise healthy adults without HIV and those living with HIV (PLWH). Methods: One-hundred and eighteen adults (65 without HIV and 53 antiretroviral-naïve PLWH), from a peri-urban setting in KwaZulu-Natal, South Africa were enrolled. IFN-γ released following stimulation with ESAT-6/CFP-10 peptides and plasma IgG antibodies specific for multiple Mtb antigens were measured using the QuantiFERON-TB Gold Plus (QFT) and customized Luminex assays, respectively. The relationships between QFT status, relative concentrations of anti-Mtb IgG, HIV-status, sex, age and CD4 count were analysed. Findings: Older age, male sex and higher CD4 count were independently associated with QFT positivity (p = 0.045, 0.05 and 0.002 respectively). There was no difference in QFT status between people with and without HIV infection (58% and 65% respectively, p = 0.06), but within CD4 count quartiles, people with HIV had higher QFT positivity than people without HIV (p = 0.008 (2nd quartile), <0.0001 (3rd quartile)). Concentrations of Mtb-specific IFN-γ were lowest, and relative concentrations of Mtb-specific IgGs were highest in PLWH in the lowest CD4 quartile. Interpretation: These results suggest that the QFT assay underestimates LTBI among immunosuppressed people with HIV and Mtb-specific IgG may be a useful alternative biomarker for Mtb infection. Further evaluation of how Mtb-specific antibodies can be leveraged to improve LTBI diagnosis is warranted, particularly in HIV-endemic areas. Fundings: NIH, AHRI, SHIP: SA-MRC and SANTHE.
