Phytosulfokine-δ: A Small Peptide, but a Big Player in Symbiosis Gene Regulation

Abstract

Nitrogen availability is one of the critical determinants of agricultural yield. Biological nitrogen fixation, such as legume–rhizobia symbiotic association, might function as a solution to fix nitrogen. Using phytosulfokine (PSK)-α sequences as a query, Yu et al., 2022 performed a comprehensive genome-wide search of legume species to identify PSK-δ, a divergent pentapeptide differing in single amino acid. Furthermore, PSK-δ exhibited nodule-specific expression with lower expression in the root, substantiating the nodule-specific temporal expression and suggesting its role in nodule development and nitrogen fixation. Additionally, in planta functional characterization in Medicago truncatula using overexpression and Tnt1-insertion mutant analysis indicated the role of PSK-δ in symbiotic nodulation. Interestingly, a similar phenotype of MtPSKδ mutant (mtpskδ) with that of wild-type control led to the hypothesis of its functional redundancy with PSK-α in nodule organogenesis. Further investigation regarding its position in the Nod-factor signaling pathway revealed the downstream function of PSK-δ in association with MtENOD11 in regulating nodule formation.

Keywords

• <i>Glycine max</i>
• <i>Lotus japonicus</i>
• <i>Medicago truncatula</i>
• phytosulfokine
• root nodule symbiosis
• small-signaling peptide