Murine brain tumor microenvironment immunophenotyping using mass cytometry

Brandon L. McClellan; Mahmoud S. Alghamri; Rohit Thalla; Pedro R. Lowenstein; Maria G. Castro

STAR Protocols (Jun 2022)

Murine brain tumor microenvironment immunophenotyping using mass cytometry

Brandon L. McClellan,
Mahmoud S. Alghamri,
Rohit Thalla,
Pedro R. Lowenstein,
Maria G. Castro

Affiliations

Brandon L. McClellan: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Corresponding author
Mahmoud S. Alghamri: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Rohit Thalla: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Pedro R. Lowenstein: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Maria G. Castro: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Corresponding author

Journal volume & issue: Vol. 3, no. 2
p. 101357

Abstract

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Summary: Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model. We describe antibody conjugation and titrations for analysis of immune cells. We then detail mouse brain tumor tissue collection and processing, staining, followed by data acquisition, analysis, and gating strategy. This protocol can be applied to any brain tumor-harboring mouse model.For complete details on the use and execution of this protocol, please refer to Alghamri et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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