Cells (Feb 2023)

Endothelial Progenitor Cells May Be Related to Major Amputation after Angioplasty in Patients with Critical Limb Ischemia

  • Daniel Santillán-Cortez,
  • Eduardo Vera-Gómez,
  • Alejandro Hernández-Patricio,
  • Atzín Suá Ruíz-Hernández,
  • Juan Ariel Gutiérrez-Buendía,
  • Karen De la Vega-Moreno,
  • Yasser Alberto Rizo-García,
  • Oscar Antonio Loman-Zuñiga,
  • Ignacio Escotto-Sánchez,
  • Juan Miguel Rodríguez-Trejo,
  • Mario Antonio Téllez-González,
  • Christian Gabriel Toledo-Lozano,
  • Tania Ortega-Rosas,
  • Silvia García,
  • Paul Mondragón-Terán,
  • Juan Antonio Suárez-Cuenca

DOI
https://doi.org/10.3390/cells12040584
Journal volume & issue
Vol. 12, no. 4
p. 584

Abstract

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Background: Critical limb ischemia represents an advanced stage of peripheral arterial disease. Angioplasty improves blood flow to the limb; however, some patients progress irreversibly to lower limb amputation. Few studies have explored the predictive potential of biomarkers during postangioplasty outcomes. Aim: To evaluate the behavior of endothelial progenitor cells in patients with critical limb ischemia, in relation to their postangioplasty outcome. Methods: Twenty patients with critical limb ischemia, candidates for angioplasty, were enrolled. Flow-mediated dilation, as well as endothelial progenitor cells (subpopulations CD45+/CD34+/CD133+/CD184+ and CD45+/CD/34+/KDR[VEGFR-2]+ estimated by flow cytometry) from blood flow close to vascular damage, were evaluated before and after angioplasty. Association with lower limb amputation during a 30-day follow-up was analyzed. Results: Endothelial progenitor cells were related with flow-mediated dilation. A higher number of baseline EPCs CD45+CD34+KDR+, as well as an impaired reactivity of endothelial progenitor cells CD45+CD34+CD133+CD184+ after angioplasty, were observed in cases further undergoing major limb amputation, with a significant discrimination ability and risk (0.75, specificity 0.83 and RR 4.5 p +CD34+KDR+, as well as an impaired reactivity of subpopulation CD45+CD34+CD133+CD184+ after angioplasty, showed a predictive ability for major limb amputation in patients with critical limb ischemia.

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