EFFECT OF DIFFERENT MODES OF THAWING ON CRYOPRESERVED SPERM MOTILITY GRASS CARP (CTENOPHARYNGODON IDELLA)

Abstract

Implementation of deep freeze genital products (cryopreservation) in fish practice the need of continuation of optimizing process of defrosting parameters. The important defines element is the proper matching of the temperature water bath and exposing inside. This research was carried out for the purpose of investigation investigate the effect of different thawing procedures for motility and fertilizing capacity of frozen/thawed grass carp (Ctenopharyngodon idella) sperm. The studies were conducted during the spawning company in 2012 at the experimental farm "Nyvka" and Institute Fisheries NAAS. The optimal mode of defrostation was conducted among nine variants of the experiment. The conditions of each experiments are determined combination of three temperature water baths: 30, 35 and 40°C and the corresponding exposures of the holding inside it with frozen sperm microtubules of grass carp: 10, 20 and 30 seconds.The best result for parameters such as total time of motion and percentage of live sperm during thawing and activated sperm were obtained at 35°C and 30 seconds of exposure. Number of live spermatozoa’s reached 77,4±0,57% and time of their movement 75±2,0 seconds. Eggs after ovulation were fertilized by received sperm for the purpose of checking of semen fertilizing capacity. Artificial fertilization was 85,6±2,8% that to confirm fertility received sperm.

Keywords

• cryopreservation
• defrosting regime
• grass carp (Ctenopharyngodon idella)
• defrosted sperm fertilizing ability
• ovulated fish eggs