Nature Communications (Oct 2020)

Genetic circuit characterization by inferring RNA polymerase movement and ribosome usage

  • Amin Espah Borujeni,
  • Jing Zhang,
  • Hamid Doosthosseini,
  • Alec A. K. Nielsen,
  • Christopher A. Voigt

DOI
https://doi.org/10.1038/s41467-020-18630-2
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 18

Abstract

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Debugging a genetic circuit is frustrated by the inability to characterize parts in the context of the circuit. Here the authors use RNA-seq and ribosome profiling to take ‘snapshots’ of a large circuit in different states.