Ability of Hypocaloric Diets to Change Nutrient–Phyla Associations after 8 Weeks of Intervention
Natália Yumi Noronha,
Luzânia dos Santos Martins,
Lígia Moriguchi Watanabe,
Marcela Augusta de Souza Pinhel,
Guilherme da Silva Rodrigues,
Isabelle Mello Schneider,
Carolina Ferreira Nicoletti,
Heitor Bernardes Pereira Delfino,
Daniela Carlos,
Carla Barbosa Nonino
Affiliations
Natália Yumi Noronha
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900-Vila Monte Alegre, Ribeirão Preto 14040-900, SP, Brazil
Luzânia dos Santos Martins
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900-Vila Monte Alegre, Ribeirão Preto 14040-900, SP, Brazil
Lígia Moriguchi Watanabe
Department of Health Sciences, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14040-900, SP, Brazil
Marcela Augusta de Souza Pinhel
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900-Vila Monte Alegre, Ribeirão Preto 14040-900, SP, Brazil
Guilherme da Silva Rodrigues
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900-Vila Monte Alegre, Ribeirão Preto 14040-900, SP, Brazil
Isabelle Mello Schneider
Department of Health Sciences, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14040-900, SP, Brazil
Carolina Ferreira Nicoletti
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900-Vila Monte Alegre, Ribeirão Preto 14040-900, SP, Brazil
Heitor Bernardes Pereira Delfino
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900-Vila Monte Alegre, Ribeirão Preto 14040-900, SP, Brazil
Daniela Carlos
Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14040-900, SP, Brazil
Carla Barbosa Nonino
Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900-Vila Monte Alegre, Ribeirão Preto 14040-900, SP, Brazil
Background: Previous studies have suggested that changes in the composition of intestinal microbiota may be influenced by dietary quality. Objective: This study aimed to assess the impact of a hypocaloric diet on the relationship between microbiota and clinical/dietary variables. Methods: This was a longitudinal study. Ten women with obesity (Obese Group, ObG) participated in an 8-week home-based hypocaloric diet intervention. Anthropometric, dietary intake, biochemical, and gut microbiota assessments were conducted before and after the intervention. Microbiota relative abundance was determined using real-time PCR in triplicate. Results: In the ObG, the hypocaloric diet intervention led to significant weight loss (before: 119.5 ± 10.3 kg; after: 114.9 ± 10.2 kg; p = 0.003). Following the intervention, positive correlations were observed between nutrient intake and phyla composition: Actinobacteria phylum with fibers (r = 0.787; p = 0.012), Firmicutes phylum with proteins (r = 0.736; p = 0.024), and Proteobacteria phylum with lipids (r = 0.669; p = 0.049). Conclusions: The hypocaloric diet intervention improved health parameters associated with obesity and its comorbidities, demonstrating associations between nutrient intake and specific phyla.
