Immunomodulatory and hepato-protective role of water based supplemented Bacillus clausii in broiler chicks

Abstract

The study aimed to evaluate the effective role of Bacillus clausii on immunity and hepato-protection in broiler chickens. Total one hundred and twenty (120) day-old broiler chicks were alienated into four groups as group-A (Control), group-B {0.01 ml/ L (4 × 106 Spore)}, group-C {0.02 ml/ L (8 × 106 Spore)} and group-D {0.03 ml/ L (12 × 106 Spore)}. Each group was consisting of 3 replicate per group (10 birds/replicate). Immunization of birds was performed according to standard vaccination schedule of broiler chicks during the trial period of 35 days. The Serum biochemical parameters analyzed were liver function tests like Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), the total proteins and alkaline phosphates (ALP). Similarly the Humoral antibody titers of Newcastle Disease (ND) Immunoglobulin-M (IgM), Immunoglobulin-G (IgG) and Immunoglobulin-A (IgA) were also determined. The results showed that Bacillus clausii supplementation @ 0.03 ml/L significantly maintained the liver biomarkers (AST, ALT and ALP) at normal level as compared to control and other treated groups. Immuno-globulins (G, M and A) level was significantly elevated with highest supplementation (0.03 ml/L). No significant (p > 0.05) changes were observed for antigen specific antibody titer against ND. In conclusion, supplementation of Bacillus clausii @ 0.03 ml/L as probiotic in drinking water significantly improved the liver function and humoralimmunity of broiler chicks.

Keywords

• Broiler production
• Probiotics
• LFT
• Cellular
• Humoral Immunity