Pro219 is an electrostatic color determinant in the light-driven sodium pump KR2

Abstract

Nakajima, Pedraza-González et al. provide a comprehensive investigation of amino acid mutations at position 219 of the sodium pump rhodopsin, KR2, and their role in the color tuning of the retinal chromophore. They prepared P219X (X= A, C, D, E, F, G, H, I, K, L, M, N, Q, R, S, T, V, W, Y) mutants of KR2, and find that all mutants are red-shifted, except for P219R, highlighting its role as a color determinant in the light-driven pump KR2.