Epidemiological Investigation, Isolation and Identification of Canine Distemper Virus in Parts of Henan and Hebei Provinces from 2019 to 2020

Abstract

【Objective】The study was carried out to investigate the prevalence and genetic diversity of canine distemper virus (CDV) in central regions of China in recent years.【Method】A total of 459 samples of dogs, minks, raccoon dogs and foxes were collected randomly from parts of Henan and Hebei provinces from 2019 to 2020. Then, RTPCR detection, isolation and identification of strains, sequencing of hemagglutinin (H) gene and phylogenetic analysis were performed.【Result】87 CDV positive samples are detected, with a positive rate of 18.96%. The positive rate of CDV in dogs is highest (37.77%) in autumn and highest in minks, raccoon dogs and foxes in July. CDV occurs in animals of different ages, and young animals are more susceptible. A wild strain is isolated and identified from the diseased samples and named HB19-1. Phylogenetic analysis of H gene sequence indicates that HB19-1 belongs to the Asia-1 genotype, and it has the closest nucleotide homology with KJ994343 from GenBank. And its nucleotide and amino acid homologous rates with the vaccine strain Onderstepoort (GeneBank Accession No.: AF378705) are 91.3% and 90.2%, respectively.【Conclusion】A CDV strain HB19-1 is isolated and obtained during the epidemiological investigation of CDV in parts of Henan and Hebei provinces from 2019 to 2020. The phylogenetic analysis indicates that HB19-1 belongs to the Asia-1 genotype and has low homology with the vaccine strain. The research results can provide a basis for the prevention and control of CDV and vaccine selection and application in parts of Henan and Hebei involved in the epidemiological investigation.

Keywords

• canine distemper
• canine distemper virus
• epidemiological investigation
• hemagglutinin
• phylogenetic analysis