Nature Communications (Dec 2022)
Next generation genetically encoded fluorescent sensors for serotonin
- Martin Kubitschke,
- Monika Müller,
- Lutz Wallhorn,
- Mauro Pulin,
- Manuel Mittag,
- Stefan Pollok,
- Tim Ziebarth,
- Svenja Bremshey,
- Jill Gerdey,
- Kristin Carolin Claussen,
- Kim Renken,
- Juliana Groß,
- Pascal Gneiße,
- Niklas Meyer,
- J. Simon Wiegert,
- Andreas Reiner,
- Martin Fuhrmann,
- Olivia Andrea Masseck
Affiliations
- Martin Kubitschke
- Synthetic Biology, University of Bremen
- Monika Müller
- Neuroimmunology and Imaging Group, German Center for Neurodegenerative Diseases (DZNE)
- Lutz Wallhorn
- Synthetic Biology, University of Bremen
- Mauro Pulin
- Synaptic Wiring and Information Processing, Center for Molecular Neurobiology (ZMNH), UKE
- Manuel Mittag
- Neuroimmunology and Imaging Group, German Center for Neurodegenerative Diseases (DZNE)
- Stefan Pollok
- Cellular Neurobiology, Department of Biology and Biotechnology, Ruhr University Bochum
- Tim Ziebarth
- Cellular Neurobiology, Department of Biology and Biotechnology, Ruhr University Bochum
- Svenja Bremshey
- Synthetic Biology, University of Bremen
- Jill Gerdey
- Synthetic Biology, University of Bremen
- Kristin Carolin Claussen
- Synthetic Biology, University of Bremen
- Kim Renken
- Synthetic Biology, University of Bremen
- Juliana Groß
- Synthetic Biology, University of Bremen
- Pascal Gneiße
- Synthetic Biology, University of Bremen
- Niklas Meyer
- Synthetic Biology, University of Bremen
- J. Simon Wiegert
- Synaptic Wiring and Information Processing, Center for Molecular Neurobiology (ZMNH), UKE
- Andreas Reiner
- Cellular Neurobiology, Department of Biology and Biotechnology, Ruhr University Bochum
- Martin Fuhrmann
- Neuroimmunology and Imaging Group, German Center for Neurodegenerative Diseases (DZNE)
- Olivia Andrea Masseck
- Synthetic Biology, University of Bremen
- DOI
- https://doi.org/10.1038/s41467-022-35200-w
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 17
Abstract
Genetically encoded sensors have been developed and become versatile tools for imaging serotonin dynamics. Here, authors developed a family of serotonin (5-HT) sensors (sDarken), three variants with different affinities for 5-HT enable high spatiotemporal resolution of 5-HT dynamics.
