Journal of Pharmacological Sciences (Jan 2003)

Effects of 4,4'-Diisothiocyanato-stilbene-2,2'-disulfonic Acid (DIDS) and Chlorpromazine on NO3- Transport via Anion Exchanger in Erythrocytes: Inertness of DIDS in Whole Blood

  • Kaname Kubota,
  • Takaharu Ishibashi,
  • Taku Matsubara,
  • Tomoyuki Hori,
  • Kazuyuki Ozaki,
  • Masaru Yamazoe,
  • Junko Yoshida,
  • Matomo Nishio,
  • Yoshifusa Aizawa

Journal volume & issue
Vol. 93, no. 4
pp. 505 – 508

Abstract

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ABSTRACT: We examined the effects of chlorpromazine on NO3- transport between erythrocytes (RBCs) and extracellular fluid. Chlorpromazine (10 μg/ml) did not influence NO3- movement in both whole blood and RBC suspension. Though an anion exchanger (AE1) inhibitor DIDS (4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid, 100 μM) did not alter NO3- movement in whole blood, it inhibited the movement in a concentration-dependent manner in the RBC suspension. The inhibition was abrogated by plasma and albumin concentration-dependently. Our results indicated that chlorpromazine had no effect on NO3- transport through AE1 and that the inertness of DIDS on AE1 in whole blood is due to interference by albumin in plasma.