Single-Cell Transcriptome Sequencing and Proteomics Reveal Neonatal Ileum Dynamic Developmental Potentials

Qingshi Meng; Liang Chen; Bohui Xiong; Beining Kang; Pengfei Zhang; Shanlong Tang; Hui Han; Wei Shen; Xiaohui Feng; Shengnan Feng; Ruqing Zhong; Xiangfang Tang; Sheng Zhang; Hongfu Zhang; Yong Zhao

doi:10.1128/mSystems.00725-21

mSystems (Oct 2021)

Single-Cell Transcriptome Sequencing and Proteomics Reveal Neonatal Ileum Dynamic Developmental Potentials

Qingshi Meng,
Liang Chen,
Bohui Xiong,
Beining Kang,
Pengfei Zhang,
Shanlong Tang,
Hui Han,
Wei Shen,
Xiaohui Feng,
Shengnan Feng,
Ruqing Zhong,
Xiangfang Tang,
Sheng Zhang,
Hongfu Zhang,
Yong Zhao

Affiliations

Qingshi Meng: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Liang Chen: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Bohui Xiong: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Beining Kang: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Pengfei Zhang: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Shanlong Tang: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Hui Han: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Wei Shen: College of Life Sciences, Qingdao Agricultural University, Qingdao, People’s Republic of China
Xiaohui Feng: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Shengnan Feng: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Ruqing Zhong: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Xiangfang Tang: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Sheng Zhang: Proteomics and Metabolomics Facility, Cornell University, Ithaca, New York, USA
Hongfu Zhang: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Yong Zhao: ORCiD; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

DOI: https://doi.org/10.1128/mSystems.00725-21
Journal volume & issue: Vol. 6, no. 5

Abstract

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We found previously unknown neonatal ileum developmental potentials: specific increases in undifferentiated cells, unique enterocyte differentiation, and time dependent reduction in secretory cells. Specific transcriptional factors (TFs), ligand-receptor pairs, G protein-coupled receptors, transforming growth factor β, bone morphogenetic protein signaling pathways, and the gut mucosal microbiota are involved in this process.

Published in mSystems

ISSN: 2379-5077 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/msystems

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