SRpHi ratiometric pH biosensors for super-resolution microscopy

Douglas S. Richardson; Carola Gregor; Franziska R. Winter; Nicolai T. Urban; Steffen J. Sahl; Katrin I. Willig; Stefan W. Hell

doi:10.1038/s41467-017-00606-4

Nature Communications (Sep 2017)

SRpHi ratiometric pH biosensors for super-resolution microscopy

Douglas S. Richardson,
Carola Gregor,
Franziska R. Winter,
Nicolai T. Urban,
Steffen J. Sahl,
Katrin I. Willig,
Stefan W. Hell

Affiliations

Douglas S. Richardson: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry
Carola Gregor: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry
Franziska R. Winter: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry
Nicolai T. Urban: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry
Steffen J. Sahl: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry
Katrin I. Willig: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry
Stefan W. Hell: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry

DOI: https://doi.org/10.1038/s41467-017-00606-4
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 9

Abstract

Read online

Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-resolution microscopy, and optimize their delivery to endosomes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal