Journal of Enzyme Inhibition and Medicinal Chemistry (Jan 2019)

Evaluation of the anticancer potential of a sulphonamide carbonic anhydrase IX inhibitor on cervical cancer cells

  • Ismail Koyuncu,
  • Yasin Tülüce,
  • Hewa Slahaddin Qadir,
  • Mustafa Durgun,
  • Claudiu T. Supuran

DOI
https://doi.org/10.1080/14756366.2019.1579805
Journal volume & issue
Vol. 34, no. 1
pp. 703 – 711

Abstract

Read online

Cervical cancer is a common type of cancer. Carbonic anhydrase IX (CA IX) is an attractive target for tumour therapy, being overexpressed in many cancers. We investigated the anticancer properties of the aromatic sulphonamide S-1 as a CA IX inhibitor on cervical cancer cells (HeLa) positive for CA IX expression and normal prostate epithelial cell line (PNT1-A) negative for CA IX. We examined the cytotoxic, apoptosis, genotoxic, and oxidative stress activity of S-1 on HeLa and PNT1-A cell lines. S-1 induced significant reduction of cell viability, caused apoptosis, and up-regulated ROS production. This decrease in cell survival rate can be attributed to the high level of ROS and apoptosis, which has also been shown to arrest the cell cycle. Our findings indicated that S-1 is more effective on HeLa than PNT1-A. S-1 was able to induce apoptosis of cervical cancer cells and is a possible candidate for future anticancer studies.

Keywords