Frontiers in Microbiology (Apr 2022)
Plasma Exosomal Proteomic Pattern of Epstein-Barr Virus-Associated Hemophagocytic Lymphohistiocytosis
- Yan Xie,
- Yan Xie,
- Yan Xie,
- Yan Xie,
- Li Yang,
- Li Yang,
- Li Yang,
- Li Yang,
- Pengfei Cao,
- Pengfei Cao,
- Shen Li,
- Shen Li,
- Shen Li,
- Shen Li,
- Wentao Zhang,
- Wentao Zhang,
- Wentao Zhang,
- Wentao Zhang,
- Wei Dang,
- Wei Dang,
- Wei Dang,
- Wei Dang,
- Shuyu Xin,
- Shuyu Xin,
- Shuyu Xin,
- Shuyu Xin,
- Mingjuan Jiang,
- Mingjuan Jiang,
- Mingjuan Jiang,
- Mingjuan Jiang,
- Yujie Xin,
- Yujie Xin,
- Yujie Xin,
- Yujie Xin,
- Jing Li,
- Jing Li,
- Jing Li,
- Jing Li,
- Sijing Long,
- Sijing Long,
- Sijing Long,
- Sijing Long,
- Yiwei Wang,
- Yiwei Wang,
- Yiwei Wang,
- Yiwei Wang,
- Senmiao Zhang,
- Senmiao Zhang,
- Senmiao Zhang,
- Senmiao Zhang,
- Yang Yang,
- Yang Yang,
- Yang Yang,
- Yang Yang,
- Jianhong Lu,
- Jianhong Lu,
- Jianhong Lu,
- Jianhong Lu
Affiliations
- Yan Xie
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Yan Xie
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Yan Xie
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Yan Xie
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Li Yang
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Li Yang
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Li Yang
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Li Yang
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Pengfei Cao
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Pengfei Cao
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Shen Li
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Shen Li
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Shen Li
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Shen Li
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Wentao Zhang
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Wentao Zhang
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Wentao Zhang
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Wentao Zhang
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Wei Dang
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Wei Dang
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Wei Dang
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Wei Dang
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Shuyu Xin
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Shuyu Xin
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Shuyu Xin
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Shuyu Xin
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Mingjuan Jiang
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Mingjuan Jiang
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Mingjuan Jiang
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Mingjuan Jiang
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Yujie Xin
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Yujie Xin
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Yujie Xin
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Yujie Xin
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Jing Li
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Jing Li
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Jing Li
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Jing Li
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Sijing Long
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Sijing Long
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Sijing Long
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Sijing Long
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Yiwei Wang
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Yiwei Wang
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Yiwei Wang
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Yiwei Wang
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Senmiao Zhang
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Senmiao Zhang
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Senmiao Zhang
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Senmiao Zhang
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Yang Yang
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Yang Yang
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Yang Yang
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Yang Yang
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- Jianhong Lu
- Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
- Jianhong Lu
- Department of Microbiology, School of Basic Medical Science, Central South University, Changsha, China
- Jianhong Lu
- National Healthcare Commission (NHC) Key Laboratory of Carcinogenesis, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, China
- Jianhong Lu
- China-Africa Research Center of Infectious Diseases, Central South University, Changsha, China
- DOI
- https://doi.org/10.3389/fmicb.2022.821311
- Journal volume & issue
-
Vol. 13
Abstract
Epstein-Barr virus (EBV)-associated hemophagocytic lymphohistiocytosis (EBV-HLH) is a life-threatening syndrome, which is caused by EBV infection that is usually refractory to treatment and shows relapse. The development of new biomarkers for the early diagnosis and clinical treatment of EBV-HLH is urgently needed. Exosomes have been shown to mediate various biological processes and are ideal non-invasive biomarkers. Here, we present the differential plasma exosomal proteome of a patient with EBV-HLH before vs. during treatment and with that of his healthy twin brother. A tandem mass tag-labeled LC-MS technique was employed for proteomic detection. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated that differential proteomic profiles were related to virus infection, coagulopathy, nervous system dysfunction, imbalance of immune response, and abnormal liver function. The candidate biomarkers were first identified in the patient’s plasma exosomes at different treatment and follow-up time points. Then, 14 additional EBV-HLH exosome samples were used to verify six differentially expressed proteins. The upregulation of C-reactive protein, moesin, galectin three-binding protein, and heat shock cognate 71 kDa protein and the downregulation of plasminogen and fibronectin 1 could serve as potential biomarkers of EBV-HLH. This plasma exosomal proteomic analysis provides new insights into the diagnostic and therapeutic biomarkers of EBV-HLH.
Keywords
