Insights into mitotic checkpoint by integrating CRISPR and RNAi

Abstract

Our recent study of the mitotic checkpoint protein BUB1 (budding uninhibited by benzimidazoles 1) revealed several apparent BUB1 knock-out cell lines expressing low levels of BUB1 protein sufficient to support spindle assembly checkpoint activity. This rings alarm bells on the application of CRISPR technology.

Keywords

• spindle assembly checkpoint
• bub1
• rod
• crispr