3D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone–implant interface in vivo

Xiao Liu; Yali Miao; Haifeng Liang; Jingjing Diao; Lijing Hao; Zhifeng Shi; Naru Zhao; Yingjun Wang

Bioactive Materials (Jun 2022)

3D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone–implant interface in vivo

Xiao Liu,
Yali Miao,
Haifeng Liang,
Jingjing Diao,
Lijing Hao,
Zhifeng Shi,
Naru Zhao,
Yingjun Wang

Affiliations

Xiao Liu: National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou, 510006, China
Yali Miao: School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China
Haifeng Liang: Department of Orthopedics, Zhujiang Hospital, Southern Medical University, No. 253 Industrial Avenue, Haizhu, Guangzhou, 510280, People's Republic of China
Jingjing Diao: National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Medical Devices Research & Testing Center of SCUT, Guangzhou, 510006, PR China
Lijing Hao: National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou, 510006, China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China
Zhifeng Shi: National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Medical Devices Research & Testing Center of SCUT, Guangzhou, 510006, PR China
Naru Zhao: National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou, 510006, China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China; Corresponding author. School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China.;
Yingjun Wang: National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou, 510006, China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China; Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), 510005 Guangzhou, China; Corresponding author. National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China.

Journal volume & issue: Vol. 12
pp. 120 – 132

Abstract

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Calcium phosphate bio-ceramics are osteo-conductive, but it remains a challenge to promote the induction of bone augmentation and capillary formation. The surface micro/nano-topography of materials can be recognized by cells and then the cell fate are mediated. Traditional regulation methods of carving surface structures on bio-ceramics employ mineral reagents and organic additives, which might introduce impurity phases and affect the biological results. In a previous study, a facile and novel method was utilized with ultrapure water as the unique reagent for hydrothermal treatment, and a uniform hydroxyapatite (HAp) surface layer was constructed on composite ceramics (β-TCP/CaSiO3) in situ. Further combined with 3D printing technology, biomimetic hierarchical structure scaffolds were fabricated with interconnected porous composite ceramic scaffolds as the architecture and micro/nano-rod hybrid HAp as the surface layer. The obtained HAp surface layer favoured cell adhesion, alleviated the cytotoxicity of precursor scaffolds, and upregulated the cellular differentiation of mBMSCs and gene expression of HUVECs in vitro. In vivo studies showed that capillary formation, bone augmentation and new bone matrix formation were upregulated after the HAp surface layer was obtained, and the results confirmed that the fabricated biomimetic hierarchical structure scaffold could be an effective candidate for bone regeneration.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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