In Vitro Antioxidant and Fibroblast Migration Activities of Fractions Eluded from Dichloromethane Leaf Extract of <i>Marantodes pumilum</i>
Abbirami Balachandran,
Stepfanie N. Siyumbwa,
Gabriele R. A. Froemming,
Morak-Młodawska Beata,
Jeleń Małgorzata,
Charlie A. Lavilla,
Merell P. Billacura,
Patrick N. Okechukwu
Affiliations
Abbirami Balachandran
Department of Biotechnology, Faculty of Applied Sciences, UCSI University, Cheras, Kuala Lumpur 56000, Selangor, Malaysia
Stepfanie N. Siyumbwa
Department of Pathology and Microbiology, School of Medicine, Lusaka P.O. Box 50110, Zambia
Gabriele R. A. Froemming
Basic Medical Sciences, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak (UNIMAS), Kota Samarahan 94300, Sarawak, Malaysia
Morak-Młodawska Beata
Faculty of Pharmaceutical Sciences, Department of Organic Chemistry, Medical University of Sílesia, Jagiellonska, Str. 4, 41-200 Sosnowiec, Poland
Jeleń Małgorzata
Faculty of Pharmaceutical Sciences, Department of Organic Chemistry, Medical University of Sílesia, Jagiellonska, Str. 4, 41-200 Sosnowiec, Poland
Charlie A. Lavilla
Chemistry Department, College of Science & Mathematics, Mindanao State University-Iligan Institute of Technology, Iligan City 9200, Lanao del Norte, Philippines
Merell P. Billacura
Department of Chemistry, College of Natural Sciences & Mathematics, Mindanao State University-Main Campus, Marawi City 9700, Lanao del Sur, Philippines
Patrick N. Okechukwu
Department of Biotechnology, Faculty of Applied Sciences, UCSI University, Cheras, Kuala Lumpur 56000, Selangor, Malaysia
(1) The complexity of diabetes and diabetic wound healing remains a therapeutic challenge because proper and systematic wound care and management are essential to prevent chronic microbial infection and mechanical damage to the skin. Marantodes pumilum, locally known as ‘Kacip Fatimah’, is an herb that has been previously reported to possess anti-inflammatory, analgesic, antinociceptive and antipyretic properties. The current study aims to assess the antioxidant and fibroblast cell migration activities of the fractions eluded from the dichloromethane extract of M. pumilum leaves. (2) The total antioxidant capacity of M. pumilum was assessed using the total proanthocyanidins and phosphomolybdenum assays, while DPPH, nitric oxide, hydrogen peroxide and superoxide free radical scavenging assays were tested to determine the antioxidant potential of M. pumilum. An in vitro scratch wound assay was performed to measure the fibroblast cell migration rate using normal and insulin-resistant human dermal fibroblast cells. (3) All M. pumilum fractions exhibited good antioxidant and fibroblast cell migration activity, among which fractions A and E displayed the greatest effect. (4) M. pumilum’s fibroblast migration activity could be attributed to its strong antioxidant properties along with its previously reported properties.
