Using Bio-Functionalized Magnetic Nanoparticles and Dynamic Nuclear Magnetic Resonance to Characterize the Time-Dependent Spin-Spin Relaxation Time for Sensitive Bio-Detection
Shu-Hsien Liao,
Kuen-Lin Chen,
Chun-Ming Wang,
Jen-Jie Chieh,
Herng-Er Horng,
Li-Min Wang,
C. H. Wu,
Hong-Chang Yang
Affiliations
Shu-Hsien Liao
Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan
Kuen-Lin Chen
Department of Electro-Optical Engineering, Kun Shan University, Tainan 710, Taiwan
Chun-Ming Wang
Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan
Jen-Jie Chieh
Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan
Herng-Er Horng
Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan
Li-Min Wang
Graduate Institute of Applied Physics and Department of Physics, National Taiwan University, Taipei 106, Taiwan
C. H. Wu
Department of Physics, National Chung Hsing University, Taichung 402, Taiwan
Hong-Chang Yang
Department of Electro-Optical Engineering, Kun Shan University, Tainan 710, Taiwan
In this work, we report the use of bio-functionalized magnetic nanoparticles (BMNs) and dynamic magnetic resonance (DMR) to characterize the time-dependent spin-spin relaxation time for sensitive bio-detection. The biomarkers are the human C-reactive protein (CRP) while the BMNs are the anti-CRP bound onto dextran-coated Fe3O4 particles labeled as Fe3O4-antiCRP. It was found the time-dependent spin-spin relaxation time, T2, of protons decreases as time evolves. Additionally, the ΔT2 of of protons in BMNs increases as the concentration of CRP increases. We attribute these to the formation of the magnetic clusters that deteriorate the field homogeneity of nearby protons. A sensitivity better than 0.1 μg/mL for assaying CRP is achieved, which is much higher than that required by the clinical criteria (0.5 mg/dL). The present MR-detection platform shows promise for further use in detecting tumors, viruses, and proteins.
