Nature Communications (Nov 2017)

Phage display and selection of lanthipeptides on the carboxy-terminus of the gene-3 minor coat protein

  • Johannes H. Urban,
  • Markus A. Moosmeier,
  • Tobias Aumüller,
  • Marcus Thein,
  • Tjibbe Bosma,
  • Rick Rink,
  • Katharina Groth,
  • Moritz Zulley,
  • Katja Siegers,
  • Kathrin Tissot,
  • Gert N. Moll,
  • Josef Prassler

DOI
https://doi.org/10.1038/s41467-017-01413-7
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 10

Abstract

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Lanthipeptides are a class of cyclic post-translationally modified peptides with potential drug-like properties. Here the authors develop a phage display system by expressing lanthipeptide precursors as C-terminal fusions to the phage M13 coat protein pIII in E. coli along with the heterologous modifying enzymes.