Frontiers in Cell and Developmental Biology (May 2022)
Isolation of Viable Single Cells With High Yield and Purity Using a Small Amount of Human Kidney Tissue Biopsy
- Hasnaa Yaigoub,
- Hasna Tirichen,
- Xiaohong Xin,
- Xiaohong Xin,
- Shuhong Shi,
- Changxin Wu,
- Rongshan Li,
- Rongshan Li,
- Yafeng Li,
- Yafeng Li,
- Yafeng Li,
- Yafeng Li
Affiliations
- Hasnaa Yaigoub
- Institutes of Biomedical Sciences, Shanxi University, Taiyuan, China
- Hasna Tirichen
- Institutes of Biomedical Sciences, Shanxi University, Taiyuan, China
- Xiaohong Xin
- Department of Nephrology, The Fifth Hospital (Shanxi Provincial People’s Hospital) of Shanxi Medical University, Taiyuan, China
- Xiaohong Xin
- Shanxi Provincial Key Laboratory of Kidney Disease, Taiyuan, China
- Shuhong Shi
- Core Laboratory, The Fifth Hospital (Shanxi Provincial People’s Hospital) of Shanxi Medical University, Taiyuan, China
- Changxin Wu
- Institutes of Biomedical Sciences, Shanxi University, Taiyuan, China
- Rongshan Li
- Department of Nephrology, The Fifth Hospital (Shanxi Provincial People’s Hospital) of Shanxi Medical University, Taiyuan, China
- Rongshan Li
- Shanxi Provincial Key Laboratory of Kidney Disease, Taiyuan, China
- Yafeng Li
- Department of Nephrology, The Fifth Hospital (Shanxi Provincial People’s Hospital) of Shanxi Medical University, Taiyuan, China
- Yafeng Li
- Shanxi Provincial Key Laboratory of Kidney Disease, Taiyuan, China
- Yafeng Li
- Academy of Microbial Ecology, Shanxi Medical University, Taiyuan, China
- Yafeng Li
- Core Laboratory, The Fifth Hospital (Shanxi Provincial People’s Hospital) of Shanxi Medical University, Taiyuan, China
- DOI
- https://doi.org/10.3389/fcell.2022.822275
- Journal volume & issue
-
Vol. 10
Abstract
Graphical Abstract
Keywords
- human kidney
- core needle biopsy
- single-cell suspension
- tissue dissociation
- enzymatic digestion
- cell purification
