Chitosan-coated iron(III) oxide nanoparticles and tungsten disulfide quantum dots-immobilized Fiber-based WaveFlex Biosensor for Staphylococcus Aureus bacterial detection in real food samples

Xianzheng Lang; Ragini Singh; Qinghua Zeng; Jun Li; Daniele Tosi; Jan Nedoma; Carlos Marques; Bingyuan Zhang; Santosh Kumar

Sensors and Actuators Reports (Dec 2024)

Chitosan-coated iron(III) oxide nanoparticles and tungsten disulfide quantum dots-immobilized Fiber-based WaveFlex Biosensor for Staphylococcus Aureus bacterial detection in real food samples

Xianzheng Lang,
Ragini Singh,
Qinghua Zeng,
Jun Li,
Daniele Tosi,
Jan Nedoma,
Carlos Marques,
Bingyuan Zhang,
Santosh Kumar

Affiliations

Xianzheng Lang: Shandong Key Laboratory of Optical Communication Science and Technology, School of Physics Science and Information Technology, Liaocheng University, Liaocheng, 252059, China
Ragini Singh: Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, 522302, India; Centre of Excellence for Nanotechnology, Department of Electronics and Communication Engineering, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, 522302, India; Corresponding authors.
Qinghua Zeng: Agricultural Science and Engineering School, Liaocheng University, Liaocheng, 252059, China
Jun Li: State Key Laboratory for Macromolecule Drugs and Large-scale Manufacturing, School of Pharmaceutical Sciences, Liaocheng University, Liaocheng 252059, China
Daniele Tosi: Department of Electrical and Computer Engineering, Nazarbayev University, 010000 Astana, Kazakhstan
Jan Nedoma: Department of Telecommunications, VSB – Technical University of Ostrava, Ostrava, 70800, Czech Republic
Carlos Marques: CICECO – Aveiro Institute of Materials, Physics Department, University of Aveiro, 3810-193, Aveiro, Portugal; Department of Physics, VSB – Technical University of Ostrava, Ostrava, 70800, Czech Republic
Bingyuan Zhang: Shandong Key Laboratory of Optical Communication Science and Technology, School of Physics Science and Information Technology, Liaocheng University, Liaocheng, 252059, China; Corresponding authors.
Santosh Kumar: Centre of Excellence for Nanotechnology, Department of Electronics and Communication Engineering, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, 522302, India; Corresponding authors.

Journal volume & issue: Vol. 8
p. 100239

Abstract

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The research proposes and investigates an extraordinarily sensitive, label-free WaveFlex biosensor utilizing optical fiber technology for the detection of Staphylococcus aureus (S. aureus), a common foodborne bacterium. The WaveFlex biosensor (plasmon Wave based Flexible optical fiber Biosensor) is based on a novel flexible tapered single-mode fiber structure, utilizing gold nanoparticles (AuNPs) to trigger the phenomenon of localized surface plasmon resonance (LSPR). Additionally, the sensitivity is further enhanced using chitosan-coated iron(III) oxide nanoparticles (Fe3O4–CS NPs) and tungsten disulfide quantum dots (WS2-QDs). The optical fiber surface is functionalized with antibodies to achieve specific detection of S. aureus. For S. aureus concentrations at 1 × 108 CFU/mL (colony-forming units per milliliter), the sensor's maximum sensitivity of 2.74 nm/lg (CFU/mL), and a detection limit (LOD) of 6.67 CFU/mL. This ultra-sensitive biosensor holds great potential for widespread applications in various fields, including disease detection, medical diagnostics, and food safety inspection.

Published in Sensors and Actuators Reports

ISSN: 2666-0539 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Mathematics: Instruments and machines
Website: https://www.journals.elsevier.com/sensors-and-actuators-reports/

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