Nature Communications (Oct 2018)

Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture

  • Monica Suryana Tjin,
  • Alvin Wen Choong Chua,
  • Aida Moreno-Moral,
  • Li Yen Chong,
  • Po Yin Tang,
  • Nathan Peter Harmston,
  • Zuhua Cai,
  • Enrico Petretto,
  • Bien Keem Tan,
  • Karl Tryggvason

DOI
https://doi.org/10.1038/s41467-018-06934-3
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 10

Abstract

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In vitro expansion of human epidermal keratinocytes to resurface severe wound defects still relies on a human/mouse xenograft culture system. Here the authors develop a fully human, xeno-free culture system using skin-associated laminins, normally present in vivo, to replace mouse feeder cells.