Establishment and comparison of different procedures for modeling intrauterine adhesion in rats: A preliminary study
Peng-Cheng Liu,
Yu-Ting Song,
Long-Mei Zhao,
Yan-Ling Jiang,
Jun-Gen Hu,
Li Dong,
Xing-li Zhou,
Li Zhou,
Yaxing Li,
Jesse Li-Ling,
Hui-Qi Xie
Affiliations
Peng-Cheng Liu
Department of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, China
Yu-Ting Song
Department of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, China
Long-Mei Zhao
Department of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, China
Yan-Ling Jiang
Department of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, China
Jun-Gen Hu
Department of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
Li Dong
Regenerative Medicine Research Center of Topregmed, Chengdu, Sichuan, China
Xing-li Zhou
Department of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, China
Li Zhou
Research Core Facility of West China Hospital, Sichuan University, Chengdu, Sichuan, China
Yaxing Li
Department of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, China
Jesse Li-Ling
Department of Medical Genetics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China
Hui-Qi Xie
Department of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, China; Corresponding author. Department of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
The establishment of a stable animal model for intrauterine adhesion (IUA) can significantly enhance research on the pathogenesis and pathological changes of this disease, as well as on the development of innovative therapeutic approaches. In this study, three different modeling methods, including phenol mucilage combined mechanical scraping, ethanol combined mechanical scraping and ethanol modeling alone were designed. The morphological characteristics of the models were evaluated. The underlying mechanisms and fertility capacity of the ethanol modeling group were analyzed and compared to those of the sham surgery group. All three methods resulted in severe intrauterine adhesions, with ethanol being identified as a reliable modeling agent and was subsequently subjected to further evaluation. Immunohistochemistry and RT-PCR results indicated that the ethanol modeling group exhibited an increase in the degree of fibrosis and inflammation, as well as a significant reduction in endometrial thickness, gland number, vascularization, and endometrial receptivity, ultimately resulting in the loss of fertility capacity. The aforementioned findings indicate that the intrauterine perfusion of 95 % ethanol is efficacious in inducing the development of intrauterine adhesions in rats. Given its cost-effectiveness, efficacy, and stability in IUA formation, the use of 95 % ethanol intrauterine perfusion may serve as a novel platform for evaluating innovative anti-adhesion materials and bioengineered therapies.
