Circulating microRNAs improve bacterial infection diagnosis and overall survival prediction in acute decompensation of liver cirrhosis
Yasmina Chouik,
Fanny Lebossé,
Marie-Laure Plissonnier,
Jean-Christophe Lega,
Pierre Pradat,
Teresa Antonini,
Miroslava Subic,
Kerstin Hartig-Lavie,
Domitille Erard,
François Villeret,
Céline Guichon,
Audrey Payancé,
Sylvie Radenne,
Pierre-Emmanuel Rautou,
Fabien Zoulim,
Massimo Levrero
Affiliations
Yasmina Chouik
Cancer Research Center of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Lyon, France; Department of Hepatology, Hôpital Croix-Rousse, Hospices Civils de Lyon, Lyon, France; University of Lyon Claude Bernard 1 (UCLB1), Lyon, France; Corresponding author
Fanny Lebossé
Cancer Research Center of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Lyon, France; Department of Hepatology, Hôpital Croix-Rousse, Hospices Civils de Lyon, Lyon, France; University of Lyon Claude Bernard 1 (UCLB1), Lyon, France
Marie-Laure Plissonnier
Cancer Research Center of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Lyon, France
Jean-Christophe Lega
Department of Internal Medicine, Hôpital Lyon Sud, Hospices Civils de Lyon, Lyon, France
Pierre Pradat
Clinical Research Center, GHN, Hospices Civils de Lyon, Lyon, France
Teresa Antonini
Department of Hepatology, Hôpital Croix-Rousse, Hospices Civils de Lyon, Lyon, France
Miroslava Subic
University of Lyon Claude Bernard 1 (UCLB1), Lyon, France
Kerstin Hartig-Lavie
Department of Hepatology, Hôpital Croix-Rousse, Hospices Civils de Lyon, Lyon, France
Domitille Erard
Department of Hepatology, Hôpital Croix-Rousse, Hospices Civils de Lyon, Lyon, France
François Villeret
Department of Hepatology, Hôpital Croix-Rousse, Hospices Civils de Lyon, Lyon, France; University of Lyon Claude Bernard 1 (UCLB1), Lyon, France
Céline Guichon
Department of Anesthesiology and Intensive Care, Hôpital Croix-Rousse, Hospices Civils de Lyon, Lyon, France
Audrey Payancé
Université Paris-Cité, Inserm, Centre de recherche sur l’inflammation, UMR 1149, Paris, France
Sylvie Radenne
Department of Hepatology, Hôpital Croix-Rousse, Hospices Civils de Lyon, Lyon, France
Pierre-Emmanuel Rautou
Université Paris-Cité, Inserm, Centre de recherche sur l’inflammation, UMR 1149, Paris, France; Service d'Hépatologie, AP-HP, Hôpital Beaujon, DMU DIGEST, Centre de Référence des Maladies Vasculaires du Foie, FILFOIE, ERN RARE-LIVER, Clichy, France
Fabien Zoulim
Cancer Research Center of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Lyon, France; Department of Hepatology, Hôpital Croix-Rousse, Hospices Civils de Lyon, Lyon, France; University of Lyon Claude Bernard 1 (UCLB1), Lyon, France
Massimo Levrero
Cancer Research Center of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Lyon, France; Department of Hepatology, Hôpital Croix-Rousse, Hospices Civils de Lyon, Lyon, France; University of Lyon Claude Bernard 1 (UCLB1), Lyon, France; Department of Medicine SCIAC and the Italian Institute of Technology (IIT) Center for Life Nanosciences (CLNS), University of Rome La Sapienza, Rome, Italy; Corresponding author
Summary: Bacterial infections are the most frequent precipitating event in patients with acute decompensation of cirrhosis (AD) and are associated with high mortality. Early diagnosis is challenging due to cirrhosis-related systemic inflammation. Here we investigated the potential of circulating microRNAs to diagnose bacterial infections and predict survival in cirrhotic patients with AD. High throughput profiling of circulating microRNAs was performed using the Nanostring technology in 57 AD patients and 24 patients with compensated cirrhosis (CC). Circulating miRs profiling showed that: (a) miRs differentially detected in AD vs. CC were mostly down-regulated; (b) a composite score including absolute neutrophil count, C reactive protein and miR-362-3p could diagnose bacterial infection with an excellent performance (AUC of 0.825 [95% CI = 0.671–0.980; p < 0.001]); (c) a composite score including miR-382-5p, miR-592 and MELD-Na improved 6-month survival prediction. Circulating miRs are strongly dysregulated in patients with AD and may help to improve bacterial infection diagnosis and survival prediction.
