Evaluation of antimicrobial efficacy of neem and Aloe vera leaf extracts in comparison with 3% sodium hypochlorite and 2% chlorhexidine against E. faecalis and C. albicans

Abstract

Aim: The aim of study was to determine the antimicrobial efficacy of alcoholic neem and Aloe vera leaf extracts against Enterococcus faecalis and Candida albicans in comparison with 3% sodium hypochlorite (3% NaOCl) and 2% chlorhexidine (2% CHX). Materials and Methods: In the present study, indigenously prepared neem and Aloe vera leaf extracts were serially diluted to determine the minimum inhibitory concentration (MIC) at which the E. faecalis and C. albicans were sensitive, and compared it with 3% NaOCl and 2% CHX by the agar-well diffusion method. Results: The MICs of the alcoholic neem extract to E. faecalis and C. albicans were determined as 0.94% and 1.88%, and for Aloe vera extracts they were 1.88% and 3.75%, respectively and the zones of diffusions that were formed around the extracts in the respective agar plates were significantly greater than with 3% NaOCl and 2% CHX within the groups when analyzed statistically by one-way analysis of variance (ANOVA). Conclusion: The antimicrobial efficacy of extracts was well demonstrated from the present in vitro observation of the agar-well diffusion method, and hence it may be advantageous if we use these extracts as irrigating solutions in endodontics. However, it requires further preclinical and clinical evaluation.

Keywords

• Agar-well diffusion method
• alcoholic neem extract
• Aloe vera extract
• C. albicans
• 2% chlorhexidine (CHX). E. faecalis
• minimum inhibitory concentration
• serial broth dilution
• 3% sodium hypochlorite (NaOCl)