Callus induction and regeneration in selected indica genotypes of rice (Oryza sativa L.)

Abstract

Plumule of four rice varieties viz., TRY 2, TRY 3, ADT 53 and CO 52 were studied for callus induction and regeneration using different concentrations and combinations of plant growth regulators in MS medium. The combination of MS medium with 2,4-D (2.0 mg/l) + Kn (0.5 mg/l) showed maximum callus induction frequency in all four varieties ranged from 93.33 to 76.67 compared to other growth regulator combinations. Upon subculture of the callus, MS medium with NAA (0.5 mg/l) + BAP (2.0 mg/l) was found to be superior for greening of calli and regeneration of shoots, followed by MS + NAA (0.5 mg/l) + BAP (1.5 mg/l). Among the four varieties TRY 2 and TRY 3 showed positive responses for regeneration.

Keywords

• rice
• in vitro culture
• plumule culture
• callus induction and regeneration