Hormonal and histological study on the protective effect of Moringa oleifera against chromium toxicity

Abstract

The current study aimed to know the effects of oral administration of Moringa oleifera leaves powder which contains the active substances to prevent the harmful toxic effect of chromium Cr (potassium dichromate; VI) on some hormonal and biochemical tests, in addition to understand the relationship between active compound of Moringa oleifera on testes and liver tissues against chromium toxicity. Thirty-three healthy male white New Zealand rabbits were randomly distributed in three groups (11 rabbits in each group). The first group were orally drenched normal saline considered as a control group. The second, were treated with chromium (40 mg kg-1 body weight/day) to notice experimental toxicity on some blood parameters and tissues structure., The third with 300 mg Moringa oleifera powder /kg diet/day with the chromium (40 mg kg-1 body weight/day). Treating for all groups was continued for 60 days. The results showed negative effects as a significant decrease (p≤ 0.01) in testosterone and inhibin-B hormone, glutathione (GSH) and total antioxidant capacity (TAC), by the increased level of FSH, alanin amino transferase (ALT), aspartate amino transferase (AST), liver cholesterol and malondialdehyde (MDA), in addition to histological variation in liver and testes in the rabbit serum dealt with chromium (group 2). In contrast, a positive effect was observed as a significant raise in testosterone, inhibin-B hormone, GSH and TAC, by decreased levels of FSH, ALT, AST, liver cholesterol and MDA in the serum dealt with Moringa oleifera (group 3), in addition to the improvement effects on testicular tissue and liver to be closed to normal concentration in control group.

Keywords

• inhibin- b
• potassium dichromate
• testosterone hormone
• tac
• gsh