Ex vivo Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery
Damayanti Chakraborty,
Masanaga Muto,
Michael Soares
Affiliations
Damayanti Chakraborty
Institute for Reproductive Health and Regenerative Medicine, Departments of Pathology & Laboratory Medicine and Pediatrics, University of Kansas Medical Center, Kansas City, KS, USA
Masanaga Muto
Institute for Reproductive Health and Regenerative Medicine, Departments of Pathology & Laboratory Medicine and Pediatrics, University of Kansas Medical Center, Kansas City, KS, USA
Michael Soares
Institute for Reproductive Health and Regenerative Medicine, Departments of Pathology & Laboratory Medicine and Pediatrics, University of Kansas Medical Center, Kansas City, KS, USAFetal Health Research, Children’s Research Institute, Children’s Mercy, Kansas City, MO, USA
In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of ‘gain-of-function’ and ‘loss-of-function’ blastocyst manipulations can be evaluated with in vitro outgrowth assays or following transfer to pseudopregnant rats.
