A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing

Affiliations

Yaojun Tong: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark
Tue S. Jørgensen: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark
Christopher M. Whitford: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark
Tilmann Weber: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark
Sang Yup Lee: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark

Abstract

CRISPR prime editing enables double-strand break free engineering of the genome. Here the authors present a toolkit for prime editing in E. coli.