Biotecnología Vegetal (Apr 2013)

<i> In vitro </i> establishment of axillary buds of <i>Colocasia esculenta</i> Schott clone `INIVIT MC-2001'

  • Diosdada Gálvez Guerra,
  • Manuel Cabrera Jova,
  • Yoel Beovides García,
  • Ania Robaina Jiménez,
  • Sergio Rodríguez Morales,
  • Daniel Rodríguez Pérez

Journal volume & issue
Vol. 13, no. 2

Abstract

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The new Colocacia esculenta Schott clone `INIVIT MC-2001', obtained by the INIVIT breeding program, presented high percentages of losses in the in vitro establishment phase, mainly by microbial contamination. The aim of this study was to establish axillary buds of taro clone `INIVIT MC-2001' with low percentages of microbial contamination and explants mortality. For disinfection, six treatments were studied using different concentrations and times of immersion in sodium hypochlorite (NaClO) and ozonized water. Furthermore, the influence of the concentration of 6-BAP (0.1, 0.3, 0.5 and 1.0 mg l-1 of 6-BAP) in the culture medium was determined. With the use of 3.0% sodium hypochlorite for the disinfection of taro corms for 25 minutes we were able to reduce the percentage of microbial contamination and gain efficiency in the establishment of the apices of axillary buds of 85.38%. By employing the culture medium consisting of MS salts and vitamins, with 0.5 mg l-1 6-BAP at 29 days of culture, the explants were achieved the morphology characteristics for transferring to the multiplication phase. Keywords: axillary buds, Colocasia, ozone.